On-farm anaerobic digestion (AD) in North America is generally located on dairy cattle farms, as they are optimal sites for AD due to dairy manure being a low-solids slurry with minimal bedding that is easily pumpable and collected daily. Lystek's patented Thermal Hydrolysis Process (Lystek THP®) uses high-speed shearing, heating, and alkali to cause hydrolysis of biological cells. Lystek THP hydrolyzes solid and liquid residuals, reducing the viscosity of feedstocks such that the final product is discharged with a total solids concentration up to 15%, but the physical properties of a pumpable liquid. LysteMize® is the process of anaerobically digesting the hydrolyzed feedstock to enhance AD through multiple mechanisms: particle size reduction, feedstock homogenization, and solubilization of recalcitrant organic carbon. Biosolids and food waste feedstocks have demonstrated that Lystek-hydrolyzed substrate will generate biogas faster than unhydrolyzed material, which allows for shorter hydraulic retention time. LysteMize is typically applied in two main configurations: 1. Upstream of a conventional digester to improve efficiency and increase capacity; or, 2. Upstream of an anaerobic LysteMize digestion tank, without the heating or mixing requirements of a conventional digester. The LysteMize Livestock Waste Characterization Study is evaluating the commercial viability of manure as feedstocks for the LysteMize digestion process by analyzing a range of samples from different livestock types, including: dairy cow, beef cow, swine, sheep, goat, and various poultry. Lystek was awarded a grant from Natural Resources Canada in 2023 and will complete all biochemical methane potential (BMP) testing and characterization in summer 2025. This paper will discuss results from the Study alongside operational considerations for manure digestion applications.